![]() ![]() The method of claim 9 wherein the recombinant host cell comprises deletion of fra2.ġ1. The method of claim 3 wherein the recombinant host cell further comprises at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide affecting Fe-S cluster biosynthesis.ġ0. The method of claim 3 wherein the recombinant host cell further comprises reduced or eliminated pyruvate decarboxylase activity.ĩ. ![]() The method of claim 6 wherein the host cell further comprises a heterologous polynucleotide encoding a polypeptide which catalyzes the substrate to product conversion isobutyraldehyde to isobutanol.Ĩ. The method of claim 3 wherein the recombinant host cell further comprises heterologous polynucleotides encoding polypeptides which catalyze the substrate to product conversions: (a) pyruvate to acetolactate (b) acetolactate to 2,3-dihydroxyisovalerate and (c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate.ħ. The method of claim 3 wherein the recombinant host cell is Saccharomyces cerevisiae.Ħ. The method of claim 3 wherein the recombinant host cell is a member of the genera Clostridium, Zymomonas, Escherichia, Salmonella, Serratia, Erwinia, Klebsiella, Shigella, Rhodococcus, Pseudomonas, Bacillus, Lactobacillus, Enterococcus, Alcaligenes, Klebsiella, Paenibacillus, Arthrobacter, Corynebacterium, Brevibacterium, Schizosaccharomyces, Issatchenkia, Kluyveromyces, Yarrowia, Pichia, Candida, Hansenula, or Saccharomyces.ĥ. The method of claim 1 wherein the contacting occurs within a recombinant host cell and wherein the polypeptide is heterologous to recombinant host cell.Ĥ. The method of claim 1 wherein the polypeptide has a KIVD cluster profile HMM E value of less than 1E-223 using the hmmsearch program.ģ. contacting said polypeptide with α-ketoisovalerate under conditions wherein isobutyraldehyde is produced.Ģ. providing a polypeptide wherein said polypeptide comprises at least one of: (i) at least 80% identity to SEQ ID NO: 51, 52, 53, 55, 56, 58, 59, 61 or 63 or an active fragment thereof or (ii) α-ketoisovalerate decarboxylase activity, a specificity ratio for α-ketoisovalerate to pyruvate greater than 1, and thiamine diphosphate cofactor activation constant (K c) of about 20 μM or less and b. ![]() A method of converting α-ketoisovalerate to isobutyraldehyde comprising a. ![]()
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